Mouse AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit
The Mouse AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit is a powerful tool meticulously crafted for the precise and quantitative assessment of AGER levels in diverse mouse tissue and biological samples.
AGER, a significant receptor belonging to the advanced glycosylation end product (AGE) receptor family, is involved in various cellular processes implicated in diabetes, inflammation, and the development of chronic complications. Our Mouse AGER ELISA Kit from Assay Genie guarantees exceptional sensitivity and specificity, ensuring the accurate quantification of AGER levels crucial for understanding its role in pathological conditions. With stringent quality control standards in place, this kit delivers robust performance and reproducible results, making it an ideal choice for researchers investigating the complex mechanisms related to advanced glycosylation end products and their impact on health and disease.
Product Name:
Mouse AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit
SKU:
AEES00213
Target:
Mouse AGER (Total Advanced Glycosylation End Product Specific Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AGER. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AGER and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AGER, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse AGER. You can calculate the concentration of Mouse AGER in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
88-104
87-96
Average (%)
95
96
90
1:4
Range (%)
87-96
95-102
90-100
Average (%)
90
98
96
1:8
Range (%)
93-104
91-98
89-99
Average (%)
100
94
96
1:16
Range (%)
94-107
92-104
97-110
Average (%)
101
96
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-104
101
EDTA plasma (n=5)
89-98
92
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.25
224.62
837.07
96.24
215.92
971.07
Standard deviation
5.21
10.92
36.25
5.77
10.36
40.69
C V (%)
5.47
4.86
4.33
6.0
4.8
4.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse AGER concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AGER in samples. No significant cross-reactivity or interference between Mouse AGER and analogues was observed.
