The Mouse Albumin (ALB) ELISA Kit is meticulously crafted for the precise quantification of Albumin levels in diverse biological samples. Albumin, a vital protein produced in the liver, serves essential roles in maintaining osmotic pressure, transporting molecules, and contributing to various physiological functions. This ELISA kit delivers unparalleled sensitivity and specificity to ensure accurate measurement of Albumin levels, enabling researchers to explore aspects related to liver function, kidney health, and metabolic processes.
With stringent quality control measures, this kit guarantees reliable performance and reproducible results, offering a valuable tool for scientific investigations and clinical research.
Product Name:
Mouse ALB (Albumin) ELISA Kit
SKU:
AEES00227
Target:
Mouse ALB (Albumin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 ng/mL
Detection range:
62.50-4000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ALB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ALB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ALB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ALB. You can calculate the concentration of Mouse ALB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-94
89-103
85-98
Average (%)
91
97
93
1:4
Range (%)
94-105
90-98
86-97
Average (%)
97
93
92
1:8
Range (%)
98-109
85-99
88-102
Average (%)
104
92
94
1:16
Range (%)
88-98
95-105
87-101
Average (%)
93
98
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
97
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
88-96
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
176.51
475.93
1938.99
183.37
432.11
1733.6
Standard deviation
9.81
22.56
99.47
10.05
20.78
78.53
C V (%)
5.56
4.74
5.13
5.48
4.81
4.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ALB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ALB in samples. No significant cross-reactivity or interference between Mouse ALB and analogues was observed.
