The Mouse ALCAM (Activated Leukocyte Cell Adhesion Molecule) ELISA Kit is a powerful tool designed for the accurate and sensitive measurement of ALCAM levels in mouse serum, plasma, and cell culture supernatants. This kit is specifically engineered to ensure high specificity and reproducibility, allowing researchers to obtain reliable results for various research applications.ALCAM, also known as CD166, is a cell adhesion molecule that plays a crucial role in immune response and inflammation. It is involved in leukocyte migration, T-cell activation, and cell-cell adhesion, making it a key player in various physiological and pathological processes.
The measurement of ALCAM levels can provide valuable insights into immune system function, inflammatory responses, and disease pathology.By utilizing the Mouse ALCAM ELISA Kit, researchers can gain a better understanding of the role of ALCAM in various biological processes and diseases, including cancer, autoimmune disorders, and inflammatory conditions. This kit offers a reliable and efficient method for quantifying ALCAM levels, facilitating further research into novel therapeutic strategies and diagnostic approaches.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ALCAM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ALCAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ALCAM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ALCAM. You can calculate the concentration of Mouse ALCAM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
92-106
86-99
Average (%)
105
97
91
1:4
Range (%)
88-102
80-92
82-96
Average (%)
94
86
88
1:8
Range (%)
95-108
87-99
86-99
Average (%)
100
93
92
1:16
Range (%)
93-103
85-99
86-100
Average (%)
98
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.04
3.83
0.44
1.11
3.8
Standard deviation
0.03
0.04
0.12
0.03
0.06
0.12
C V (%)
6.52
3.85
3.13
6.82
5.41
3.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ALCAM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ALCAM in samples. No significant cross-reactivity or interference between Mouse ALCAM and analogues was observed.
