Description
Mouse ALPL (Alkaline Phosphatase,Liver/Bone/Kidney) ELISA Kit
The Mouse ALPL (Alkaline Phosphatase, Liver/Bone/Kidney) ELISA Kit is a specialized assay designed for the quantitative detection of Alkaline Phosphatase (ALP) levels in biological samples obtained from mice.
Alkaline Phosphatase is a crucial enzyme found in various tissues, including the liver, bone, and kidney, and plays a key role in several physiological processes, such as bone mineralization, phosphate metabolism, and liver function. Accurate measurement of ALP levels is essential for studying bone health, liver diseases, and kidney function in mouse models. The Mouse ALPL ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance, delivering accurate quantification of ALP levels in mouse samples. Trust Assay Genie's Mouse ALPL ELISA Kit for precise and dependable analysis of Alkaline Phosphatase in your research studies.
| Product Name: | Mouse ALPL (Alkaline Phosphatase,Liver/Bone/Kidney) ELISA Kit |
| Product Code: | AEES00194 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 0.16-10 ng/mL |
| Sensitivity: | 0.09 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Mouse ALPL in samples. No significant cross-reactivity or interference between Mouse ALPL and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse ALPL concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ALPL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ALPL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ALPL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ALPL. You can calculate the concentration of Mouse ALPL in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
