The Mouse Anti-NMDAR Antibody ELISA Kit is specifically designed for the precise detection of NMDAR (N-methyl-D-aspartic acid receptor) antibodies in mouse serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications. NMDAR antibodies play a critical role in modulating NMDAR function, which is essential for synaptic plasticity, learning, and memory. Dysregulation of NMDAR signaling has been implicated in various neurological and psychiatric disorders, highlighting the importance of studying NMDAR antibodies as potential biomarkers for these conditions.
Whether investigating neurodevelopmental disorders, neurodegenerative diseases, or autoimmune encephalitis, the Mouse Anti-NMDAR Antibody ELISA Kit provides researchers with a valuable tool for uncovering novel insights into the pathophysiology of these conditions and developing new therapeutic strategies.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
0
1
2
3
Serum (n = 5)
92-101%
87-98%
83-97%
EDTA Plasma (n = 5)
85-103%
83-101%
82-89%
Heparin Plasma (n = 5)
88-98%
84-100%
80-94%
Recovery:
0
1
2
Serum (n = 5)
85-102
91
EDTA Plasma (n = 5)
88-102
96
Heparin Plasma (n = 5)
85-104
98
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Biotin-Labeled Antigen Binding: Add biotin-labeled antigen working solution and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add SABC working solution and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes until color develops.
6
Stop Reaction & Reading: Add stop solution and read absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes and collect supernatant.
Plasma
Collect in anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue with PBS or lysis buffer, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells on ice using lysis buffer, centrifuge and collect supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
