The Assay Genie Mouse ApoB100 (Apolipoprotein B100) ELISA Kit is expertly developed to enable the precise and quantitative measurement of Apolipoprotein B100 (ApoB100) levels within various biological samples sourced from mouse models. ApoB100 is a critical component of lipoproteins involved in lipid metabolism and cardiovascular health, making the accurate quantification of its levels essential for studying lipid transport, atherosclerosis, and related metabolic disorders. Our Mouse ApoB100 ELISA Kit offers exceptional sensitivity and specificity, guaranteeing reliable and reproducible results crucial for assessing ApoB100's role in lipid regulation and cardiovascular function.
Manufactured under rigorous quality control standards, this kit is designed to deliver robust performance while remaining user-friendly, providing an excellent tool for conducting research studies focused on elucidating the functions of Apolipoprotein B100 in mouse models. Trust the Assay Genie Mouse ApoB100 ELISA Kit for accurate and dependable quantification of this important biomarker in your research endeavors.
Product Name:
Mouse ApoB100 (Apolipoprotein B100) ELISA Kit
SKU:
AEES00188
Target:
Mouse ApoB100 (Apolipoprotein B100)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 ng/mL
Detection range:
78.13-5000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoB100. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoB100 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoB100, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ApoB100. You can calculate the concentration of Mouse ApoB100 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
94-108
88-98
Average (%)
105
101
93
1:4
Range (%)
98-115
83-94
92-103
Average (%)
106
89
98
1:8
Range (%)
98-115
83-96
96-114
Average (%)
105
88
104
1:16
Range (%)
95-108
84-99
91-103
Average (%)
100
91
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
88-104
96
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
236.1
536.8
1740.6
251.9
503.0
1759.0
Standard deviation
14.6
27.4
57.4
15.6
24.1
75.6
C V (%)
6.18
5.1
3.3
6.19
4.79
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ApoB100 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ApoB100 in samples. No significant cross-reactivity or interference between Mouse ApoB100 and analogues was observed.
