The Mouse ASGR1 (Asialoglycoprotein Receptor 1) CLIA Kit is specifically designed for the accurate and reliable detection of ASGR1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ASGR1 is a key protein involved in the recognition and clearance of circulating glycoproteins, playing a crucial role in liver function and overall metabolism. Dysregulation of ASGR1 has been linked to various liver diseases and metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
The Mouse ASGR1 CLIA Kit from Assay Genie provides researchers with a powerful tool for investigating the role of ASGR1 in health and disease, offering a comprehensive solution for accurate and quantitative analysis of ASGR1 levels in mouse samples. Trust Assay Genie for high-quality, reliable results in your research endeavors.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ASGR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ASGR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ASGR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ASGR1. You can calculate the concentration of Mouse ASGR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
85-100
88-103
Average (%)
101
91
95
1:4
Range (%)
89-101
101-115
92-106
Average (%)
95
107
100
1:8
Range (%)
103-119
96-111
91-104
Average (%)
110
103
97
1:16
Range (%)
85-100
96-114
91-103
Average (%)
92
104
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-112
102
EDTA plasma (n=5)
96-110
103
Cell culture media (n=5)
94-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.97
109.64
386.71
35.55
105.79
415.65
Standard deviation
3.71
9.76
39.83
4.59
12.42
28.39
C V (%)
9.77
8.9
10.3
12.91
11.74
6.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ASGR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ASGR1 in samples. No significant cross-reactivity or interference between Mouse ASGR1 and analogues was observed.
