The Mouse BMF (BCL-2 Modifying Factor) CLIA Kit is specifically designed for the quantitative measurement of BMF levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.BMF, a key regulator of apoptosis, plays a critical role in modulating the BCL-2 family proteins, leading to cell death or survival. Dysregulation of BMF expression has been associated with various diseases, including cancer, autoimmune disorders, and neurodegenerative diseases, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic interventions.
Overall, the Mouse BMF CLIA Kit is a reliable tool for researchers seeking to explore the role of BMF in health and disease, providing accurate and meaningful data for their investigations.
Product Name:
Mouse BMF (Bcl-2 Modifying Factor) CLIA Kit
SKU:
MOES00114
Target:
Mouse BMF (Bcl-2 Modifying Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BMF. You can calculate the concentration of Mouse BMF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
102-118
89-102
Average (%)
108
108
95
1:4
Range (%)
96-107
95-109
97-113
Average (%)
101
101
103
1:8
Range (%)
98-110
93-105
95-110
Average (%)
103
98
102
1:16
Range (%)
99-115
90-102
102-119
Average (%)
107
96
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
97-112
102
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
196.8
338.73
1863.85
181.28
354.15
1918.35
Standard deviation
20.27
26.01
112.58
16.93
32.51
157.88
C V (%)
10.3
7.68
6.04
9.34
9.18
8.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BMF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BMF in samples. No significant cross-reactivity or interference between Mouse BMF and analogues was observed.
