The Mouse CAR (Nr1i3) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of CAR (Constitutive Androstane Receptor) levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for various research applications. CAR is a nuclear receptor that plays a crucial role in regulating drug metabolism and detoxification pathways in the liver. Its activation can lead to the induction of various drug-metabolizing enzymes, making it an important factor in drug efficacy and toxicity.
Studying CAR levels can provide valuable insights into the metabolism of xenobiotics and potential drug interactions. This ELISA kit is an essential tool for researchers studying drug metabolism, toxicology, and liver function in mouse models. Its high sensitivity and specificity make it a valuable resource for investigating the role of CAR in various physiological and pathological conditions.
Product Name:
Mouse CAR/NR1I3 ELISA Kit
SKU:
MOFI00541
Reactivity:
Mouse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-96%
84-98%
83-98%
EDTA Plasma (n = 5)
91-103%
83-99%
89-95%
Heparin Plasma (n = 5)
85-105%
82-97%
87-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-105
97
EDTA Plasma (n = 5)
87-104
96
Heparin Plasma (n = 5)
91-105
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
