Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit
The Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit is a specialized assay designed to quantitatively measure the levels of MCP-5 in various biological samples sourced from mouse models. MCP-5, also known as CCL12, is a chemokine known for its role in immune responses and monocyte chemotaxis, making it a key mediator in inflammatory processes and immune cell recruitment. This ELISA kit by Assay Genie is tailored to offer exceptional sensitivity and specificity, ensuring accurate and reproducible quantification of MCP-5 levels.
With stringent quality control measures in place and validated protocols, researchers can rely on this kit for robust performance and precise results. The user-friendly design of the kit further enhances its usability, making it a valuable tool for research aimed at understanding the functions and implications of MCP-5 in inflammatory responses and related biological processes. Trust in Assay Genie's Mouse CCL12/MCP-5 ELISA Kit for reliable and insightful data in your research endeavors.
Product Name:
Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit
SKU:
AEES00200
Target:
Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCL12/MCP-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CCL12/MCP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCL12/MCP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CCL12/MCP-5. You can calculate the concentration of Mouse CCL12/MCP-5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
97-105
91-103
Average (%)
95
102
97
1:4
Range (%)
94-107
88-103
84-94
Average (%)
100
95
91
1:8
Range (%)
86-95
97-105
94-104
Average (%)
92
102
98
1:16
Range (%)
82-97
93-105
90-104
Average (%)
90
100
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
100-110
103
Cell culture media (n=5)
84-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
173.22
443.46
1632.31
168.36
471.43
1765.51
Standard deviation
9.16
20.67
72.47
8.96
22.72
91.63
C V (%)
5.29
4.66
4.44
5.32
4.82
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CCL12/MCP-5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CCL12/MCP-5 in samples. No significant cross-reactivity or interference between Mouse CCL12/MCP-5 and analogues was observed.
