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PE/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody [RA3.3A 1/6.1] (AGEL1414)

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SKU AGEL1414
Product Type Antibody
Antibody Type Monoclonal Antibody
Applications FC
Reactivity Mouse
Host Species Rat
Isotype IgG2a,k
Clone RA3.3A 1/6.1
Conjugation PE/Cy5.5
€100 - €275
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Description

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PE/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody [RA3.3A 1/6.1]

The PE-Cyanine5.5 Conjugated Mouse CD45R/B220 Monoclonal Antibody (RA3-3A1/6.1) is a specialized tool for researchers studying immune cells in mice. This monoclonal antibody is designed to target the CD45R/B220 protein marker, which is commonly used to identify B cells in the immune system. By conjugating PE-Cyanine5.5 to the antibody, it allows for easy detection and analysis of B cells in flow cytometry experiments.The RA3-3A1/6.1 antibody is highly specific and has been validated for use in various applications, including flow cytometry and immunofluorescence.

Its binding to the CD45R/B220 protein provides researchers with a reliable tool for studying B cell populations in mouse models. This antibody is essential for studies focusing on B cell development, function, and response to various stimuli.By using the PE-Cyanine5.5 Conjugated Mouse CD45R/B220 Monoclonal Antibody (RA3-3A1/6.1) in your research, you can accurately characterize and investigate B cell populations in mouse samples, enhancing your understanding of immune responses and contributing to advancements in immunology and related fields.

Product Title:PE/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody [RA3.3A 1/6.1]
SKU:AGEL1414
Size:25µg, 100µg
Clone No:RA3.3A 1/6.1
Synonyms:B220;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgM, κ
Form:Liquid
Conjugation:PE/Cyanine 5.5
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:Spectrum Image
Gene ID:19264,5788
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

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