The Mouse Chem (Chemerin) ELISA Kit is specifically designed for the precise measurement of chemerin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Chemerin is a key protein involved in inflammatory responses and metabolism regulation. It plays a crucial role in conditions such as obesity, type 2 diabetes, and inflammatory diseases, making it a valuable biomarker for studying these diseases and exploring potential treatments.
Overall, the Mouse Chem (Chemerin) ELISA Kit from Assay Genie provides an efficient and reliable tool for researchers to investigate the role of chemerin in physiological and pathological processes in mouse models.
Product Name:
Mouse CHEM (Chemerin) ELISA Kit
SKU:
MOES00636
Target:
Mouse CHEM (Chemerin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
28.13 pg/mL
Detection range:
46.88-3000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CHEM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CHEM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CHEM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CHEM. You can calculate the concentration of Mouse CHEM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
84-96
94-109
Average (%)
99
91
102
1:4
Range (%)
88-101
81-94
86-100
Average (%)
94
86
94
1:8
Range (%)
88-103
81-91
86-101
Average (%)
95
86
93
1:16
Range (%)
91-102
84-97
85-97
Average (%)
97
89
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
144.72
403.28
1471.13
151.53
438.02
1470.45
Standard deviation
7.76
20.2
59.73
7.86
18.22
49.7
C V (%)
5.36
5.01
4.06
5.19
4.16
3.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CHEM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CHEM in samples. No significant cross-reactivity or interference between Mouse CHEM and analogues was observed.
