Mouse COL1 alpha2 (Collagen Type I Alpha 2) CLIA Kit
The Mouse Col1 Alpha2 (Collagen Type I Alpha 2) CLIA Kit is specifically designed for the quantitative measurement of collagen type I alpha 2 levels in mouse samples. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Collagen type I alpha 2 is a key component of the extracellular matrix and plays a crucial role in tissue structure and function. Dysregulation of collagen type I alpha 2 has been implicated in various diseases, including fibrosis, arthritis, and skin disorders.
As such, this kit serves as a valuable tool for studying collagen metabolism and its role in disease pathology.With its user-friendly protocol and robust performance, the Mouse Col1 Alpha2 CLIA Kit is a valuable asset for researchers investigating the role of collagen type I alpha 2 in physiological and pathological processes in mouse models.
Product Name:
Mouse COL1 alpha2 (Collagen Type I Alpha 2) CLIA Kit
SKU:
MOES00190
Target:
Mouse COL1 alpha2 (Collagen Type I Alpha 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL1α2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL1α2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL1α2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse COL1α2. You can calculate the concentration of Mouse COL1α2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
97-111
88-100
Average (%)
102
104
95
1:4
Range (%)
91-103
100-116
87-101
Average (%)
97
108
93
1:8
Range (%)
96-108
86-99
94-107
Average (%)
102
92
102
1:16
Range (%)
90-104
93-107
101-114
Average (%)
97
98
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
101
EDTA plasma (n=5)
99-113
106
Cell culture media (n=5)
99-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.23
260.0
678.63
90.99
248.33
730.76
Standard deviation
11.79
18.49
76.07
11.05
20.49
54.22
C V (%)
12.0
7.11
11.21
12.14
8.25
7.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse COL1α2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL1α2 in samples. No significant cross-reactivity or interference between Mouse COL1α2 and analogues was observed.
