Mouse COL4 alpha1 (Collagen Type IV Alpha 1) CLIA Kit
The Mouse COL4 Alpha1 (Collagen Type IV Alpha 1) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of mouse COL4 Alpha1 levels in various biological samples. This kit is ideal for researchers looking to study the role of COL4 Alpha1 in extracellular matrix formation and cell adhesion processes.COL4 Alpha1 is a key component of Type IV Collagen, a major structural protein in basement membranes. Dysregulation of COL4 Alpha1 has been implicated in various diseases, including kidney diseases, autoimmune disorders, and certain types of cancer.
By accurately measuring COL4 Alpha1 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.The Mouse COL4 Alpha1 CLIA Kit offers reliable and reproducible results, making it suitable for a wide range of research applications in the fields of molecular biology, cell biology, and pathology. With its high sensitivity and specificity, this kit is a valuable tool for investigating the role of COL4 Alpha1 in health and disease.
Product Name:
Mouse COL4 alpha1 (Collagen Type IV Alpha 1) CLIA Kit
SKU:
MOES00191
Target:
Mouse COL4 alpha1 (Collagen Type IV Alpha 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL4α1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL4α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL4α1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse COL4α1. You can calculate the concentration of Mouse COL4α1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
103-117
95-106
Average (%)
94
109
100
1:4
Range (%)
103-116
87-100
99-113
Average (%)
109
92
107
1:8
Range (%)
95-108
96-113
87-99
Average (%)
102
103
92
1:16
Range (%)
90-105
100-119
103-118
Average (%)
97
108
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
107
EDTA plasma (n=5)
93-106
101
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.6
237.89
942.32
105.29
254.76
956.5
Standard deviation
12.25
25.98
84.9
12.2
26.88
58.35
C V (%)
11.18
10.92
9.01
11.59
10.55
6.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse COL4α1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL4α1 in samples. No significant cross-reactivity or interference between Mouse COL4α1 and analogues was observed.
