The Mouse CCL27 (CTACK) Cutaneous T-Cell Attracting Chemokine CLIA Kit is specifically designed for the precise measurement of CCL27 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.CCL27, also known as Cutaneous T-Cell Attracting Chemokine, plays a critical role in immune response and skin inflammation. It attracts T-cells to the skin, regulating immune cell migration and activation.
Elevated CCL27 levels have been linked to various skin disorders and inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Mouse CCL27 (CTACK) Cutaneous T-Cell Attracting Chemokine CLIA Kit is a reliable tool for researchers looking to investigate the role of CCL27 in immune function and inflammatory diseases in mouse models. Its high quality and precision make it an essential addition to any laboratory conducting research in this area.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTACK. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTACK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTACK, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CTACK. You can calculate the concentration of Mouse CTACK in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
86-100
94-109
Average (%)
92
93
101
1:4
Range (%)
87-101
101-117
86-100
Average (%)
93
107
91
1:8
Range (%)
97-112
90-105
89-100
Average (%)
104
97
95
1:16
Range (%)
88-100
98-111
92-104
Average (%)
95
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
108
EDTA plasma (n=5)
98-112
104
Cell culture media (n=5)
99-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.9
155.04
379.83
48.22
153.34
345.19
Standard deviation
5.15
18.4
25.33
5.22
15.66
23.02
C V (%)
9.92
11.87
6.67
10.83
10.21
6.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CTACK concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTACK in samples. No significant cross-reactivity or interference between Mouse CTACK and analogues was observed.
