The Mouse Cys-C (Cystatin C) CLIA Kit is specifically designed for the quantitative detection of cystatin C levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for a variety of research applications.Cystatin C is a vital protein that plays a crucial role in regulating the activity of cysteine proteases and maintaining normal physiological functions.
Abnormal levels of cystatin C have been linked to various conditions such as kidney diseases, cardiovascular diseases, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse Cys-C CLIA Kit offers researchers a reliable tool for studying the role of cystatin C in disease pathogenesis and exploring its potential as a therapeutic target.
Product Name:
Mouse Cys-C (Cystatin C) CLIA Kit
SKU:
MOES00216
Target:
Mouse Cys-C (Cystatin C)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Cys-C. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Cys-C. You can calculate the concentration of Mouse Cys-C in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
90-104
101-114
Average (%)
101
95
107
1:4
Range (%)
87-101
102-117
99-109
Average (%)
94
110
104
1:8
Range (%)
93-105
93-107
88-99
Average (%)
99
100
93
1:16
Range (%)
91-106
99-113
90-103
Average (%)
97
106
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
105
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.35
281.96
916.38
99.43
301.18
981.29
Standard deviation
12.54
24.25
79.91
11.5
33.31
108.73
C V (%)
12.25
8.6
8.72
11.57
11.06
11.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Cys-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Cys-C in samples. No significant cross-reactivity or interference between Mouse Cys-C and analogues was observed.
