The Mouse D-LDH (D-Lactate Dehydrogenase) ELISA Kit is specially designed for the precise measurement of D-LDH levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.D-Lactate Dehydrogenase plays a vital role in the conversion of D-lactate to pyruvate in the glycolytic pathway. Abnormal levels of D-LDH have been associated with various metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.
With a user-friendly protocol and quick assay time, the Mouse D-LDH ELISA Kit provides researchers with a reliable tool for investigating D-LDH levels in biological samples. Whether studying metabolic diseases, tissue ischemia, or microbial infections, this kit is essential for precise and efficient analysis.
Product Name:
Mouse D-LDH (D-Lactate Dehydrogenase) ELISA Kit
SKU:
MOES00965
Target:
Mouse D-LDH (D-Lactate Dehydrogenase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
2.81 mIU/mL
Detection range:
4.69-300 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse D-LDH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse D-LDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse D-LDH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse D-LDH. You can calculate the concentration of Mouse D-LDH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
94-109
97-112
Average (%)
99
101
104
1:4
Range (%)
93-110
87-103
91-109
Average (%)
101
94
99
1:8
Range (%)
96-108
84-99
91-104
Average (%)
103
91
98
1:16
Range (%)
96-113
85-96
91-104
Average (%)
103
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
92-109
100
Cell culture media (n=5)
92-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
16.1
35.7
136.4
16.2
38.0
141.8
Standard deviation
1
1.5
4.2
1.1
1.6
6.7
C V (%)
6.21
4.2
3.08
6.79
4.21
4.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse D-LDH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse D-LDH in samples. No significant cross-reactivity or interference between Mouse D-LDH and analogues was observed.
