The Mouse DNA Methyltransferase 1 (DNMT1) ELISA Kit is specifically designed for the accurate quantification of DNMT1 levels in mouse samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an excellent tool for a wide range of research applications.DNMT1 is a key enzyme involved in the maintenance of DNA methylation patterns, playing a crucial role in gene expression regulation and epigenetic modifications. Dysregulation of DNMT1 has been associated with various diseases, including cancer, neurological disorders, and developmental abnormalities.
Therefore, measuring DNMT1 levels can provide valuable insights into disease mechanisms and potential therapeutic strategies.Overall, the Mouse DNA Methyltransferase 1 (DNMT1) ELISA Kit offers a powerful and versatile solution for researchers studying epigenetics, gene regulation, and disease pathogenesis in mouse models. Its ease of use and high performance make it an essential tool for advancing scientific discoveries and improving our understanding of molecular mechanisms underlying various health conditions.
Product Name:
Mouse DNA Methyltransferase 1 (DNMT1) ELISA Kit
Product Code:
MODL00385
Size:
96 Assays
Target:
DNMT1
Synonyms:
CXXC9, DNMT, MCMT, Cytosine-5 DNA Methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
