Description
Mouse ET-1 (Endothelin 1) ELISA Kit
The Mouse ET-1 (Endothelin 1) ELISA Kit is specifically designed for the quantitative detection of Endothelin 1 levels in various biological samples collected from mouse models. Endothelin 1 is a crucial vasoconstrictor peptide involved in regulating blood vessel function, blood pressure, and vascular tone. Dysregulation of Endothelin 1 has been implicated in various cardiovascular diseases and conditions, making accurate measurement of its levels vital for understanding vascular pathophysiology and disease mechanisms in mouse research models.
Assay Genie's Mouse ET-1 ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance, providing researchers with a reliable tool for the accurate quantification of Endothelin 1 levels in mice. Trust Assay Genie's Mouse ET-1 ELISA Kit for dependable and insightful analysis of this critical vascular peptide in your research studies.
| Product Name: | Mouse ET-1 (Endothelin 1) ELISA Kit |
| Product Code: | AEES00195 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 0.78-50 pg/mL |
| Sensitivity: | 0.47 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Mouse ET-1 in samples. No significant cross-reactivity or interference between Mouse ET-1 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse ET-1 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ET-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ET-1. You can calculate the concentration of Mouse ET-1 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
