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PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody [CI:A3-1] (AGEL0573)

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SKU AGEL0573
Product Type Antibody
Antibody Type Monoclonal Antibody
Applications FC
Reactivity Mouse
Host Species Rat
Isotype IgG2a,k
Clone CI:A3-1
Conjugation PerCP/Cy5.5
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Description

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PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody [CI:A3-1]

The Mouse F4/80 Monoclonal Antibody (PerCP-Cyanine5.5 Conjugated, CI:A3-1, AGEL0573) offered by Assay Genie is a highly specific antibody designed for research involving the F4/80 protein, a well-established marker for mouse macrophages. This antibody, a monoclonal antibody raised against mouse cells, is conjugated with PerCP-Cyanine5.5 for easy detection and analysis.F4/80 is a transmembrane glycoprotein expressed on the surface of mature macrophages, making it a valuable marker for studies related to macrophage identification and characterization. By specifically binding to the F4/80 protein, this antibody enables the precise detection and analysis of macrophages in various tissues and experimental settings.

Understanding the role of macrophages in immune responses, inflammation, and disease processes is essential for advancing research in immunology, cancer biology, and various other fields. The Mouse F4/80 Monoclonal Antibody provided by Assay Genie offers researchers a reliable tool for investigating the presence and function of macrophages in different biological contexts. Its high specificity and conjugation with PerCP-Cyanine5.5 make it a valuable asset for studies aiming to elucidate the complex roles of macrophages in health and disease.

Product Title:PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody [CI:A3-1]
SKU:AGEL0573
Size:25µg, 100µg
Clone No:CI:A3-1
Synonyms:Adhesion G protein-coupled receptor E1;Adgre1;Cell surface glycoprotein F4/80;EGF-like module receptor 1;Adgre1;Emr1; Gpf480;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG2b, κ
Isotype Control:PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2]
Form:Liquid
Conjugation:PerCP/Cyanine 5.5
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:Spectrum Image
Swissprot:Q61549
Gene ID:13733
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

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