Mouse Family With Sequence Similarity 3, Member B (FAM3B) ELISA Kit
The Mouse FAM3B (Family with Sequence Similarity 3 Member B) ELISA Kit is specifically designed for the precise and accurate detection of FAM3B levels in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing dependable and replicable results for a diverse array of research applications.FAM3B is a vital protein that belongs to the FAM3 family and plays a crucial role in various biological processes such as inflammation, cell proliferation, and metabolism regulation. Dysregulation of FAM3B has been implicated in several diseases, including diabetes, obesity, and cancer, making it a valuable biomarker for investigating these conditions and potentially developing therapeutic strategies.
The Mouse FAM3B ELISA Kit provides researchers with a powerful tool for studying the function and significance of FAM3B in preclinical models, offering insights into its role in disease pathology and potential as a therapeutic target. Order your kit today to advance your research and uncover new discoveries in the field of molecular biology.
Product Name:
Mouse Family With Sequence Similarity 3, Member B (FAM3B) ELISA Kit
Product Code:
MODL00450
Size:
96 Assays
Target:
FAM3B
Synonyms:
ORF9, PANDER, PRED44, C21orf11, C21orf76, Cytokine-like protein 2-21, Pancreatic-derived factor
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
