The Mouse FGF23 (Fibroblast Growth Factor 23) ELISA Kit is a powerful tool for the quantitative measurement of FGF23 levels in mouse serum, plasma, and tissue lysate samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications. FGF23 is a key regulator of phosphate and vitamin D metabolism, and abnormalities in its levels have been implicated in various diseases, including chronic kidney disease and disorders of mineral metabolism.
By accurately measuring FGF23 levels, researchers can gain valuable insights into the pathophysiology of these conditions and explore potential therapeutic interventions. With its user-friendly protocol and reliable performance, the Mouse FGF23 ELISA Kit from AssayGenie is an essential tool for researchers investigating the role of FGF23 in health and disease.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FGF23. You can calculate the concentration of Mouse FGF23 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
97-112
88-99
Average (%)
104
103
94
1:4
Range (%)
89-105
84-94
83-93
Average (%)
96
89
88
1:8
Range (%)
89-104
86-101
85-97
Average (%)
97
92
92
1:16
Range (%)
86-97
79-91
84-95
Average (%)
92
85
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
94-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.5
88.07
434.38
49.92
90.33
426.98
Standard deviation
2.52
3.69
22.37
2.53
5.18
23.27
C V (%)
5.54
4.19
5.15
5.07
5.73
5.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FGF23 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FGF23 in samples. No significant cross-reactivity or interference between Mouse FGF23 and analogues was observed.
