The Mouse FGL2 (Fibrinogen-Like Protein 2) CLIA Kit is a powerful tool for measuring levels of FGL2 in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and precise results with high sensitivity and specificity, making it an excellent choice for researchers in various fields.FGL2 is a key protein involved in immune regulation and has been implicated in a range of diseases, including autoimmune disorders and cancer. By accurately quantifying FGL2 levels, researchers can gain insights into disease mechanisms and potentially identify new therapeutic targets.
Overall, the Mouse FGL2 CLIA Kit from Assay Genie is a valuable tool for studying the role of FGL2 in health and disease, offering reliable data to drive groundbreaking research and advancements in the field. Visit the link for more information: www.assaygenie.com/mouse-fgl2-fibrinogen-like-protein-2-clia-kit-moes00261/
Product Name:
Mouse FGL2 (Fibrinogen Like Protein 2) CLIA Kit
SKU:
MOES00261
Target:
Mouse FGL2 (Fibrinogen Like Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FGL2. You can calculate the concentration of Mouse FGL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
84-99
88-99
Average (%)
101
91
94
1:4
Range (%)
101-117
91-103
99-111
Average (%)
107
96
106
1:8
Range (%)
95-109
96-109
93-107
Average (%)
100
104
100
1:16
Range (%)
87-100
100-115
99-112
Average (%)
92
107
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
106
EDTA plasma (n=5)
99-113
105
Cell culture media (n=5)
100-112
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.63
72.71
167.54
23.76
68.02
156.1
Standard deviation
3.01
5.55
12.9
2.4
6.25
17.53
C V (%)
11.74
7.63
7.7
10.1
9.19
11.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FGL2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FGL2 in samples. No significant cross-reactivity or interference between Mouse FGL2 and analogues was observed.
