The Mouse FN (Fibronectin) ELISA Kit is specifically designed for the precise measurement of fibronectin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research purposes.Fibronectin is a key component of the extracellular matrix and plays a crucial role in cell adhesion, migration, and tissue remodeling. It is involved in various physiological and pathological processes, including wound healing, embryonic development, and cancer progression.
By accurately measuring fibronectin levels, researchers can better understand its role in these processes and potentially identify new therapeutic targets.Overall, the Mouse FN (Fibronectin) ELISA Kit provides researchers with a reliable and efficient method to study the role of fibronectin in health and disease, ultimately advancing our knowledge and potentially leading to new treatments.
Product Name:
Mouse FN (Fibronectin) ELISA Kit
SKU:
MOES01029
Target:
Mouse FN (Fibronectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FN. You can calculate the concentration of Mouse FN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-112
93-107
97-109
Average (%)
106
99
103
1:4
Range (%)
91-105
84-98
88-99
Average (%)
98
90
94
1:8
Range (%)
87-100
82-93
81-96
Average (%)
94
87
88
1:16
Range (%)
89-100
86-97
82-92
Average (%)
94
91
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.82
12.64
41.91
4.42
11.94
37.91
Standard deviation
0.3
0.56
1.88
0.28
0.6
1.65
C V (%)
6.22
4.43
4.49
6.33
5.03
4.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FN in samples. No significant cross-reactivity or interference between Mouse FN and analogues was observed.
