The Mouse FVII (Coagulation Factor VII) ELISA Kit is specially designed for the precise measurement of Coagulation Factor VII levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it perfect for various research purposes.Coagulation Factor VII is a key player in the blood clotting process, essential for maintaining hemostasis and preventing excessive bleeding. Dysregulation of Coagulation Factor VII can lead to various bleeding disorders and thrombotic events, emphasizing the importance of studying its levels in different physiological and pathological conditions.
Researchers can rely on this ELISA kit to accurately quantify Coagulation Factor VII levels in mouse samples, enabling them to explore its role in coagulation disorders, cardiovascular diseases, and other related conditions. Trust in this kit to deliver insightful data for your research needs.
Product Name:
Mouse FVII (Coagulation Factor VII) ELISA Kit
SKU:
MOES01782
Target:
Mouse FVII (Coagulation Factor VII) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse F7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse F7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse F7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse F7. You can calculate the concentration of Mouse F7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-99
86-101
97-113
Average (%)
90
92
105
1:4
Range (%)
85-97
86-97
84-97
Average (%)
92
91
89
1:8
Range (%)
91-102
84-96
84-96
Average (%)
96
88
89
1:16
Range (%)
86-100
79-89
89-103
Average (%)
92
85
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
92
EDTA plasma (n=5)
95-110
100
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.07
4.7
0.48
1.06
4.76
Standard deviation
0.03
0.05
0.24
0.03
0.06
0.16
C V (%)
6
4.67
5.11
6.25
5.66
3.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse F7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse F7 in samples. No significant cross-reactivity or interference between Mouse F7 and analogues was observed.
