Mouse GCP-2 (Granulocyte Chemotactic Protein 2) CLIA Kit
The Mouse GCP-2 (Granulocyte Chemotactic Protein-2) CLIA Kit is a powerful tool for the precise measurement of GCP-2 levels in mouse biological samples. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.GCP-2 is a key chemotactic protein involved in the recruitment and activation of neutrophils, playing a critical role in the immune response to inflammation and infection.
Its levels can be indicative of various inflammatory conditions and diseases, making it a valuable biomarker for studying immune responses and developing potential therapeutics.Overall, the Mouse GCP-2 CLIA Kit offers researchers a reliable method for quantifying GCP-2 levels in mouse samples, providing valuable insights into the role of this protein in immune function and disease pathology.
Product Name:
Mouse GCP-2 (Granulocyte Chemotactic Protein 2) CLIA Kit
SKU:
MOES00310
Target:
Mouse GCP-2 (Granulocyte Chemotactic Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GCP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GCP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GCP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GCP-2. You can calculate the concentration of Mouse GCP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
101-117
100-112
Average (%)
100
107
107
1:4
Range (%)
97-113
95-112
94-108
Average (%)
105
102
101
1:8
Range (%)
93-109
84-99
96-110
Average (%)
100
91
102
1:16
Range (%)
85-98
92-105
91-106
Average (%)
92
100
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
91-101
96
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.14
68.47
348.75
41.93
62.4
362.61
Standard deviation
4.33
5.35
38.47
4.34
7.19
22.48
C V (%)
11.06
7.81
11.03
10.35
11.52
6.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GCP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GCP-2 in samples. No significant cross-reactivity or interference between Mouse GCP-2 and analogues was observed.
