Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
The Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit is meticulously designed for the quantitative detection of GDNF in various mouse biological samples. GDNF, a potent neurotrophic factor, is critically involved in promoting the growth and survival of neurons, particularly dopaminergic neurons, and plays a key role in neuronal development and maintenance. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for researchers investigating the functions and therapeutic potential of GDNF in various neurological processes.
Manufactured under strict quality control standards, this kit provides reliable performance, making it an excellent choice for neurobiology research and the study of neurotrophic factors.
Product Name:
Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
SKU:
AEES00223
Target:
Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GDNF. You can calculate the concentration of Mouse GDNF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-102
95-105
87-96
Average (%)
99
102
92
1:4
Range (%)
88-96
86-94
88-98
Average (%)
93
91
94
1:8
Range (%)
84-95
94-105
83-97
Average (%)
91
98
90
1:16
Range (%)
95-106
91-101
93-104
Average (%)
103
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-106
102
EDTA plasma (n=5)
89-103
97
Cell culture media (n=5)
87-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.91
122.61
436.32
42.69
106.79
431.17
Standard deviation
2.12
5.97
21.38
2.2
4.84
18.63
C V (%)
5.18
4.87
4.9
5.15
4.53
4.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GDNF in samples. No significant cross-reactivity or interference between Mouse GDNF and analogues was observed.
