The Mouse GH (Growth Hormone) ELISA Kit is specifically designed for the precise measurement of growth hormone levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an ideal tool for various research applications.Growth hormone is a key hormone that plays a vital role in regulating growth, metabolism, and other physiological processes in mice. It is essential for normal development and body maintenance. Monitoring growth hormone levels can provide valuable insights into growth disorders, aging, and metabolic conditions, making it a valuable biomarker for studying these conditions and exploring potential therapeutic strategies.With the Mouse GH ELISA Kit, researchers can confidently analyze growth hormone levels in mouse samples with precision and efficiency, advancing their understanding of growth regulation and related pathologies.
Product Name:
Mouse GH (Growth Hormone) ELISA Kit
SKU:
MOES00672
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
