The Mouse GHR ELISA Kit is expertly designed for the precise quantitative assessment of Growth Hormone Receptor (GHR) levels in a variety of biological samples. GHR, the receptor for growth hormone, plays a crucial role in mediating the actions of growth hormone in various cellular and physiological processes essential for growth, metabolism, and development. Accurate quantification of GHR is imperative for understanding its function in growth regulation, metabolic control, and cellular signaling pathways. By measuring GHR levels, researchers can elucidate the molecular mechanisms underlying growth hormone activity and its impacts on cellular proliferation, differentiation, and hormone response.
With exceptional sensitivity and specificity, the Mouse GHR ELISA Kit offered by Assay Genie ensures precise and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and user-friendly operation, making it an ideal solution for investigative studies aimed at unraveling the intricate functions of the Growth Hormone Receptor in growth and metabolic processes.
Product Name:
Mouse GHR (Growth Hormone Receptor) ELISA Kit
SKU:
AEES00253
Target:
Mouse GHR (Growth Hormone Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GHR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GHR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GHR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GHR. You can calculate the concentration of Mouse GHR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
96-107
96-113
Average (%)
102
102
104
1:4
Range (%)
99-115
93-103
98-112
Average (%)
105
98
105
1:8
Range (%)
98-113
93-106
93-107
Average (%)
103
101
101
1:16
Range (%)
90-104
95-105
95-109
Average (%)
97
100
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
91-108
99
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.37
168.91
791.14
100.61
155.65
764.12
Standard deviation
6.78
8.29
41.14
6.96
9.34
24.22
C V (%)
6.43
4.91
5.2
6.92
6.0
3.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GHR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GHR in samples. No significant cross-reactivity or interference between Mouse GHR and analogues was observed.
