The Mouse GLP-1 (Glucagon-Like Peptide 1) CLIA Kit (MOES00069) is a highly sensitive and specific assay designed for the accurate detection of GLP-1 levels in mouse serum, plasma, and cell culture supernatants. This kit is essential for researchers studying metabolic disorders, diabetes, and obesity, as GLP-1 plays a key role in regulating glucose homeostasis and insulin secretion.GLP-1 is a crucial hormone involved in enhancing glucose-stimulated insulin secretion, promoting satiety, and inhibiting glucagon secretion.
Dysregulation of GLP-1 levels is associated with various metabolic diseases, making it an important biomarker for understanding and developing treatments for these conditions.With its high sensitivity and reproducibility, the Mouse GLP-1 CLIA Kit is a valuable tool for researchers looking to accurately quantify GLP-1 levels in mouse samples, leading to insights into the role of this hormone in metabolic pathways and potential therapeutic interventions.
Product Name:
Mouse GLP-1 (Glucagon Like Peptide 1) CLIA Kit
SKU:
MOES00069
Target:
Mouse GLP-1 (Glucagon Like Peptide 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GLP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GLP-1. You can calculate the concentration of Mouse GLP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
101-119
92-108
Average (%)
103
110
99
1:4
Range (%)
96-110
101-118
99-114
Average (%)
104
108
106
1:8
Range (%)
88-99
85-99
86-98
Average (%)
94
91
92
1:16
Range (%)
88-102
103-116
87-101
Average (%)
93
109
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
102
EDTA plasma (n=5)
88-98
93
Cell culture media (n=5)
94-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.83
42.11
175.02
25.05
39.52
192.1
Standard deviation
2.79
4.8
13.77
3.1
4.63
14.47
C V (%)
12.22
11.4
7.87
12.38
11.72
7.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GLP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GLP-1 in samples. No significant cross-reactivity or interference between Mouse GLP-1 and analogues was observed.
