The Mouse GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) CLIA Kit is specifically designed for the precise measurement of GM-CSF levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.GM-CSF is a critical cytokine that regulates the production and function of white blood cells, playing a key role in the immune response to infections, inflammation, and autoimmune diseases.
Dysregulation of GM-CSF has been linked to various pathological conditions, making it a valuable biomarker for studying and potentially treating these diseases.With the Mouse GM-CSF CLIA Kit, researchers can confidently measure GM-CSF levels in experimental samples, gaining valuable insights into the immune response and disease mechanisms in mouse models. This kit provides a reliable tool for advancing research in immunology, oncology, and other fields where GM-CSF is a key player.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GM-CSF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GM-CSF. You can calculate the concentration of Mouse GM-CSF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
93-107
91-106
Average (%)
106
100
97
1:4
Range (%)
87-101
99-115
94-108
Average (%)
93
107
99
1:8
Range (%)
94-109
89-99
85-98
Average (%)
101
94
90
1:16
Range (%)
93-104
98-114
89-103
Average (%)
99
104
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-112
102
EDTA plasma (n=5)
99-112
106
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.01
75.56
204.21
23.35
78.97
196.17
Standard deviation
2.79
9.07
21.91
2.71
6.44
17.81
C V (%)
11.62
12.0
10.73
11.61
8.15
9.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GM-CSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GM-CSF in samples. No significant cross-reactivity or interference between Mouse GM-CSF and analogues was observed.
