The Mouse GP4 (CD36) Platelet Membrane Glycoprotein IV CLIA Kit (MOES00181) is a state-of-the-art assay designed for the precise measurement of GP4 levels in mouse plasma and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an invaluable tool for a variety of research applications.GP4, also known as CD36, is a key protein involved in platelet activation and adhesion, playing a crucial role in hemostasis and thrombosis.
Dysregulation of GP4 has been linked to various cardiovascular disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.In summary, the Mouse GP4 (CD36) Platelet Membrane Glycoprotein IV CLIA Kit (MOES00181) offers researchers a reliable and efficient method for analyzing GP4 levels in mouse samples, shedding light on the mechanisms underlying platelet function and providing insights into cardiovascular health.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GP4/CD36. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GP4/CD36 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GP4/CD36, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GP4/CD36. You can calculate the concentration of Mouse GP4/CD36 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
89-103
92-106
Average (%)
93
95
98
1:4
Range (%)
98-114
91-106
99-112
Average (%)
104
97
106
1:8
Range (%)
91-106
85-99
89-103
Average (%)
98
90
95
1:16
Range (%)
97-111
101-112
87-98
Average (%)
102
106
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
98-116
107
Cell culture media (n=5)
98-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.62
442.31
1889.84
183.63
481.92
1852.16
Standard deviation
18.19
40.91
142.12
19.61
46.55
161.14
C V (%)
9.16
9.25
7.52
10.68
9.66
8.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GP4/CD36 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GP4/CD36 in samples. No significant cross-reactivity or interference between Mouse GP4/CD36 and analogues was observed.
