The Mouse GTF2H1 (General Transcription Factor IIH Polypeptide 1) CLIA Kit is specifically designed for the accurate quantification of GTF2H1 levels in mouse serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.GTF2H1 is a vital protein involved in transcription initiation and repair of DNA damage. It plays a crucial role in gene regulation and maintaining genomic integrity. Dysregulation of GTF2H1 has been linked to various diseases, including cancer and genetic disorders, making it a valuable biomarker for studying disease mechanisms and potential therapeutic targets.
With the Mouse GTF2H1 CLIA Kit, researchers can uncover new insights into the function of GTF2H1 in mouse models and advance their understanding of transcriptional regulation and DNA repair processes. This kit is an essential tool for investigating the role of GTF2H1 in health and disease.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GTF2H1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GTF2H1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GTF2H1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GTF2H1. You can calculate the concentration of Mouse GTF2H1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
100-112
94-105
Average (%)
92
106
100
1:4
Range (%)
91-103
86-99
96-112
Average (%)
98
93
103
1:8
Range (%)
100-111
91-105
88-101
Average (%)
106
97
93
1:16
Range (%)
89-103
92-105
100-114
Average (%)
96
99
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
106
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
98-112
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
199.84
502.06
1407.06
203.21
489.21
1350.16
Standard deviation
23.56
52.87
123.26
17.27
44.52
96.81
C V (%)
11.79
10.53
8.76
8.5
9.1
7.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GTF2H1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GTF2H1 in samples. No significant cross-reactivity or interference between Mouse GTF2H1 and analogues was observed.
