The Mouse HNRPA1 (Heterogeneous Nuclear Ribonucleoprotein A1) CLIA Kit is specifically designed for the quantitative detection of HNRPA1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for research purposes.HNRPA1 is a key protein in RNA metabolism and has been linked to various cellular processes, including gene expression regulation and RNA splicing. Dysregulation of HNRPA1 has been associated with diseases such as cancer and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.
The Mouse HNRPA1 CLIA Kit is user-friendly and allows for easy and efficient detection of HNRPA1 levels, making it an ideal tool for researchers in the field of molecular biology and biomedicine. Purchase this kit today to advance your research and gain valuable insights into the role of HNRPA1 in different disease pathways.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HNRPA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HNRPA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HNRPA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HNRPA1. You can calculate the concentration of Mouse HNRPA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
96-110
97-111
Average (%)
107
102
105
1:4
Range (%)
98-112
101-115
89-102
Average (%)
103
109
94
1:8
Range (%)
98-114
93-105
89-100
Average (%)
105
98
94
1:16
Range (%)
103-119
100-115
86-96
Average (%)
110
106
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
90
EDTA plasma (n=5)
86-102
93
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.21
133.18
443.87
52.57
122.83
401.74
Standard deviation
6.41
9.34
37.42
4.67
9.89
25.35
C V (%)
12.52
7.01
8.43
8.88
8.05
6.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HNRPA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HNRPA1 in samples. No significant cross-reactivity or interference between Mouse HNRPA1 and analogues was observed.
