The Mouse Heme Oxygenase 1 (HO1) ELISA Kit is specifically designed to quantitatively detect HO1 levels in various biological samples of mice. Heme Oxygenase 1, an enzyme involved in heme catabolism, plays a crucial role in cellular defense against oxidative stress and is implicated in various physiological and pathological processes. This ELISA kit offers high sensitivity and specificity, ensuring accurate measurement of HO1 levels for research related to oxidative stress, cellular responses, and disease mechanisms.
Manufactured under stringent quality control standards, this kit ensures robust performance and reliable results, making it an excellent choice for scientific investigations and therapeutic development.
Product Name:
Mouse HO1 (Heme Oxygenase 1) ELISA Kit
SKU:
AEES00226
Target:
Mouse HO1 (Heme Oxygenase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HO1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HO1. You can calculate the concentration of Mouse HO1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-107
89-101
99-106
Average (%)
102
97
103
1:4
Range (%)
90-100
88-98
92-105
Average (%)
97
93
99
1:8
Range (%)
96-106
99-109
97-108
Average (%)
102
103
101
1:16
Range (%)
87-94
93-104
100-111
Average (%)
91
101
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
90-102
95
Cell culture media (n=5)
93-99
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
181.72
443.35
1928.05
184.96
480.68
1671.26
Standard deviation
9.9
20.53
92.16
11.1
22.02
71.03
C V (%)
5.45
4.63
4.78
6.0
4.58
4.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse HO1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HO1 in samples. No significant cross-reactivity or interference between Mouse HO1 and analogues was observed.
