The Mouse IFN-gamma (Interferon-gamma) ELISA Kit is specifically designed for the accurate measurement of IFN-gamma levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.IFN-gamma is a key cytokine involved in the immune response, playing a crucial role in the activation of immune cells and inflammation. It is essential for the defense against viruses, bacteria, and tumors. Abnormal levels of IFN-gamma have been linked to autoimmune diseases, infectious diseases, and cancer, making it a valuable biomarker for studying these conditions and developing potential treatments. With its reliable performance and ease of use, the Mouse IFN-gamma ELISA Kit is a valuable tool for researchers studying the immune system, inflammation, and related diseases in mouse models.
Product Name:
Mouse IFN-gamma (Interferon Gamma) ELISA Kit
SKU:
MOES00667
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
