The Mouse IgA (Immunoglobulin A) ELISA Kit is specifically designed for the quantitative detection of IgA levels in mouse serum, plasma, and other biological fluids. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Immunoglobulin A is an important component of the immune system, providing mucosal immunity and protection against pathogens. Elevated levels of IgA can indicate infections, autoimmune diseases, and inflammatory conditions, making it a valuable biomarker for monitoring immune response and disease progression.
With this ELISA kit, researchers can easily measure IgA levels in mouse samples, providing valuable insights into the immune system function and disease mechanisms. Whether studying autoimmune disorders, infectious diseases, or inflammatory conditions, the Mouse IgA ELISA Kit is an essential tool for advancing research in immunology and related fields.
Product Name:
Mouse IgA (Immunoglobulin A) ELISA Kit
SKU:
MOES01187
Target:
Mouse IgA (Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgA. You can calculate the concentration of Mouse IgA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
100-114
98-111
Average (%)
105
106
105
1:4
Range (%)
93-106
84-97
88-103
Average (%)
100
89
94
1:8
Range (%)
92-106
82-94
88-100
Average (%)
97
87
93
1:16
Range (%)
90-102
86-97
85-97
Average (%)
96
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
93-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.95
52.87
156.46
20.01
53.52
164.75
Standard deviation
1.44
2.29
8.53
1.08
3.18
7.51
C V (%)
6.87
4.33
5.45
5.4
5.94
4.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgA in samples. No significant cross-reactivity or interference between Mouse IgA and analogues was observed.
