The Mouse IgE (Immunoglobulin E) CLIA Kit is specifically designed for the quantitative measurement of IgE levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and precision, providing accurate and reproducible results for a variety of research applications.Immunoglobulin E is an important antibody involved in allergic responses, triggering the release of inflammatory mediators in response to allergens. Elevated IgE levels are associated with conditions such as asthma, eczema, and allergic rhinitis, making this kit an invaluable tool for studying allergic diseases and developing targeted therapies. With its easy-to-use protocol and reliable performance, the Mouse IgE CLIA Kit from Assay Genie is a valuable addition to any research laboratory focused on immunology, allergy, and inflammation. Order now to advance your research and uncover new insights into the role of IgE in allergic diseases.
Product Name:
Mouse IgE (Immunoglobulin E) CLIA Kit
SKU:
MOES00376
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
