The Mouse IgG (Immunoglobulin G) CLIA Kit is a highly sensitive and specific assay designed for the quantification of mouse IgG levels in biological samples. This kit provides accurate and reproducible results, making it suitable for a variety of research applications.Immunoglobulin G is an important antibody involved in the immune response, playing a key role in recognizing and neutralizing pathogens. Measuring IgG levels in biological samples can provide valuable insights into the immune status and response to infections or diseases.
With its high sensitivity and specificity, the Mouse IgG CLIA Kit is an essential tool for researchers studying immune responses, infectious diseases, autoimmune disorders, and vaccine development. Its ease of use and reliability make it a valuable addition to any laboratory conducting immunological research.
Product Name:
Mouse IgG (Immunoglobulin G) CLIA Kit
SKU:
MOES00377
Target:
Mouse IgG (Immunoglobulin G)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IgG. You can calculate the concentration of Mouse IgG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
92-109
91-105
Average (%)
105
100
97
1:4
Range (%)
93-108
86-100
92-104
Average (%)
98
92
97
1:8
Range (%)
100-119
98-111
97-114
Average (%)
108
105
104
1:16
Range (%)
90-103
96-108
92-106
Average (%)
96
101
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
95
EDTA plasma (n=5)
96-111
103
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
2.46
8.77
0.96
2.3
8.36
Standard deviation
0.09
0.2
0.96
0.08
0.24
0.63
C V (%)
8.49
8.13
10.95
8.33
10.43
7.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IgG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgG in samples. No significant cross-reactivity or interference between Mouse IgG and analogues was observed.
