The Mouse IgG1 (Immunoglobulin G1) CLIA kit is specifically designed for the quantitative detection of IgG1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides highly accurate and reliable results, with a high level of sensitivity and specificity, making it an ideal tool for a variety of research applications.Immunoglobulin G1 is an important subclass of antibodies that plays a crucial role in immune responses, particularly in antibody-mediated immunity.
Measurement of IgG1 levels can provide valuable insights into the immune status and response in various disease conditions and experimental models.With its ease of use and robust performance, the Mouse IgG1 CLIA kit from AssayGenie is a valuable tool for researchers studying immune responses, autoimmune diseases, infectious diseases, and antibody therapies in mouse models. Get accurate and reproducible results with this high-quality assay kit.
Product Name:
Mouse IgG1 (Immunoglobulin G1) CLIA Kit
SKU:
MOES00378
Target:
Mouse IgG1 (Immunoglobulin G1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 μg/mL
Detection range:
0.16-10 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IgG1. You can calculate the concentration of Mouse IgG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
86-98
98-113
Average (%)
108
92
103
1:4
Range (%)
95-109
96-111
88-103
Average (%)
101
104
96
1:8
Range (%)
95-111
99-113
91-104
Average (%)
103
104
97
1:16
Range (%)
98-111
103-118
97-112
Average (%)
106
109
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
99-111
105
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.52
4.25
0.6
1.5
4.45
Standard deviation
0.07
0.16
0.47
0.06
0.11
0.41
C V (%)
12.73
10.53
11.06
10.0
7.33
9.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IgG1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgG1 in samples. No significant cross-reactivity or interference between Mouse IgG1 and analogues was observed.
