The Mouse IgM (Immunoglobulin M) CLIA Kit is specifically designed for the quantitative measurement of IgM levels in mouse serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Immunoglobulin M is a key component of the immune system, playing a crucial role in the body's defense against pathogens and infections.
Monitoring IgM levels can provide valuable insights into immune function, autoimmune diseases, and inflammatory conditions.With its user-friendly protocol and reliable performance, the Mouse IgM CLIA Kit from Assay Genie is an essential tool for researchers studying immune responses and developing new therapeutic strategies. Trust in Assay Genie's expertise and high-quality products for your research needs.
Product Name:
Mouse IgM (Immunoglobulin M) CLIA Kit
SKU:
MOES00380
Target:
Mouse IgM (Immunoglobulin M)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IgM. You can calculate the concentration of Mouse IgM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
85-98
89-102
Average (%)
91
91
95
1:4
Range (%)
84-99
89-104
99-115
Average (%)
91
96
107
1:8
Range (%)
100-114
86-99
101-116
Average (%)
106
93
106
1:16
Range (%)
92-106
88-100
103-119
Average (%)
98
94
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.48
56.43
172.62
20.56
54.33
155.62
Standard deviation
1.83
6.42
16.49
2.18
4.17
14.16
C V (%)
8.94
11.38
9.55
10.6
7.68
9.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IgM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgM in samples. No significant cross-reactivity or interference between Mouse IgM and analogues was observed.
