The Mouse IL-1α ELISA Kit is a powerful tool tailored to quantitatively measure Interleukin-1 alpha (IL-1α) levels in diverse biological samples with precision and accuracy. IL-1α, an essential pro-inflammatory cytokine, plays a pivotal role in orchestrating various cellular functions, including cell proliferation, differentiation, and apoptosis. Accurate quantification of IL-1α is crucial for unraveling its intricate involvement in immune responses, inflammatory reactions, and its association with diverse inflammatory conditions, autoimmune diseases, and infections.
Understanding the levels of IL-1α is fundamental in deciphering its contributions to disease mechanisms and in formulating targeted therapeutic approaches. With exceptional sensitivity and specificity, the Mouse IL-1α ELISA Kit from Assay Genie enables researchers to obtain reliable and reproducible results. Manufactured under stringent quality control measures, this ELISA kit assures robust performance and ease of use, making it an excellent choice for research endeavors focused on exploring the multifaceted roles of IL-1α in immune regulation and inflammatory processes.
Product Name:
Mouse IL-1alpha ELISA Kit
SKU:
AEES00250
Target:
Mouse IL-1α
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 pg/mL
Detection range:
0.78-50 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-1α. You can calculate the concentration of Mouse IL-1α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
87-99
91-105
Average (%)
96
92
97
1:4
Range (%)
91-104
88-100
92-103
Average (%)
96
93
98
1:8
Range (%)
91-107
83-94
87-100
Average (%)
98
89
92
1:16
Range (%)
88-98
84-95
91-107
Average (%)
93
89
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.46
4.2
24.59
2.65
3.8
23.9
Standard deviation
0.14
0.17
0.74
0.16
0.19
1.28
C V (%)
5.83
4.01
3.01
6.14
4.89
5.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-1α in samples. No significant cross-reactivity or interference between Mouse IL-1α and analogues was observed.
