The Mouse IL-12 CLIA Kit is a reliable assay designed for the quantitative detection of IL-12 levels in mouse serum, plasma, and tissue homogenates. This kit boasts high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.IL-12 is a key cytokine involved in the regulation of immune responses, particularly in the development of T cells and natural killer cells. Dysregulation of IL-12 has been implicated in autoimmune disorders, infectious diseases, and inflammatory conditions, highlighting its significance as a potential biomarker for studying these diseases and evaluating therapeutic interventions. With the Mouse IL-12 CLIA Kit, researchers can confidently measure IL-12 levels in mouse samples, paving the way for further insights into the role of this cytokine in immune system function and disease pathology.
Product Name:
Mouse IL-12 CLIA Kit
SKU:
MOES00407
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
