The Mouse IL-13 ELISA Kit is specially designed for the precise quantification of interleukin-13 (IL-13) levels in mouse serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and consistent results, making it an indispensable tool for various research applications.IL-13 is a key cytokine that plays a vital role in modulating immune responses and inflammation. It is implicated in a variety of diseases, including asthma, allergic reactions, and autoimmune disorders, making it a valuable biomarker for understanding these conditions and developing targeted treatments. This ELISA kit provides researchers with a reliable and efficient method to measure IL-13 levels in mouse samples, facilitating in-depth analysis of immune responses and inflammatory processes in preclinical studies. Trust in the Mouse IL-13 ELISA Kit for robust and trustworthy data in your research endeavors.
Product Name:
Mouse IL-13 ELISA Kit
SKU:
MOES01222
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
