The Mouse IL1RA (Interleukin 1 Receptor Antagonist) ELISA Kit is a powerful tool for accurately measuring IL1RA levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides dependable and reproducible results, making it an excellent choice for a variety of research applications.IL1RA is a key regulatory protein that helps control inflammation by blocking the activity of interleukin-1 (IL-1), a pro-inflammatory cytokine. Dysregulation of IL1RA has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and infectious diseases. As such, measuring IL1RA levels can provide valuable insights into the underlying mechanisms of these diseases and aid in the development of targeted therapies. The Mouse IL1RA ELISA Kit from Assay Genie is a valuable tool for researchers studying inflammation, immune responses, and related pathways in mouse models. With its precise and reliable results, this kit can help advance our understanding of IL1RA biology and its role in disease pathogenesis.
Product Name:
Mouse IL-1ra ELISA Kit
SKU:
MOES00658
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
97-115
85-100
90-106
Average (%)
105
91
97
1:4
Range (%)
89-103
81-95
82-96
Average (%)
95
87
88
1:8
Range (%)
91-104
80-92
84-94
Average (%)
98
86
89
1:16
Range (%)
92-108
83-97
85-101
Average (%)
99
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
94-106
100
Cell Culture Media (n=8)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.41
320.65
843.83
94.4
302.7
856.08
Standard deviation
4.95
13.92
51.73
5.97
22.7
60.95
C V (%)
4.83
4.34
6.13
6.32
7.5
7.12
Uniport ID:
P25085
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Mouse IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Mouse IL-1ra/IL-1F3 and analogues was observed
