The Mouse IL-23 CLIA Kit (MOES00412) is a highly sensitive and specific assay designed for the accurate detection of Interleukin-23 levels in mouse serum, plasma, and cell culture supernatants. IL-23 is a key cytokine involved in inflammatory responses and immune regulation, playing a crucial role in conditions such as autoimmune diseases, inflammatory bowel disease, and cancer.This CLIA kit offers reliable and reproducible results, making it an ideal tool for research applications in immunology and inflammation.
By measuring IL-23 levels, researchers can gain valuable insights into the mechanisms underlying various diseases and potentially identify new therapeutic targets. Upgrade your research with the Mouse IL-23 CLIA Kit (MOES00412) from Assay Genie.
Product Name:
Mouse IL-23 CLIA Kit
SKU:
MOES00412
Target:
Mouse IL-23
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-23. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-23, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-23. You can calculate the concentration of Mouse IL-23 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-120
89-102
86-100
Average (%)
109
96
93
1:4
Range (%)
101-114
95-107
91-103
Average (%)
107
102
97
1:8
Range (%)
95-113
98-112
86-101
Average (%)
103
104
93
1:16
Range (%)
99-118
89-103
84-97
Average (%)
107
95
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
104
EDTA plasma (n=5)
92-108
100
Cell culture media (n=5)
100-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.64
58.91
176.63
24.05
53.32
177.19
Standard deviation
1.91
5.22
11.04
2.61
5.63
18.16
C V (%)
8.08
8.86
6.25
10.85
10.56
10.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-23 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-23 in samples. No significant cross-reactivity or interference between Mouse IL-23 and analogues was observed.
