The Mouse IL-25 (Interleukin 25) CLIA Kit is a cutting-edge assay developed for the precise measurement of IL-25 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and accuracy, ensuring consistent and trustworthy results for a variety of research purposes.IL-25, also known as IL-17E, is a key cytokine involved in regulating immune responses, particularly in allergic inflammation and autoimmune diseases.
Its role in promoting type 2 immune responses and inducing Th2 cytokine production makes it a valuable target for studying inflammatory conditions and developing novel therapeutic interventions.With its advanced technology and reliable performance, the Mouse IL-25 CLIA Kit is a valuable tool for researchers seeking to explore the complex mechanisms underlying immune responses and inflammatory diseases in mouse models.
Product Name:
Mouse IL-25 CLIA Kit
SKU:
MOES00119
Target:
Mouse IL-25
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-25. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-25 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-25, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-25. You can calculate the concentration of Mouse IL-25 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-96
93-103
95-109
Average (%)
90
98
102
1:4
Range (%)
98-110
93-105
85-95
Average (%)
104
99
90
1:8
Range (%)
89-103
98-113
86-99
Average (%)
95
105
93
1:16
Range (%)
99-114
97-108
94-109
Average (%)
107
102
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
98
EDTA plasma (n=5)
92-105
98
Cell culture media (n=5)
100-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.89
72.08
223.85
27.29
67.26
222.83
Standard deviation
2.57
7.29
17.26
2.87
4.96
25.54
C V (%)
9.93
10.11
7.71
10.52
7.37
11.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-25 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-25 in samples. No significant cross-reactivity or interference between Mouse IL-25 and analogues was observed.
