Mouse Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit
The Mouse KEAP1 (Kelch-like ECH-associated protein 1) ELISA Kit is a highly reliable and sensitive tool for the quantitative measurement of KEAP1 levels in mouse samples. This kit is specifically designed for the detection of KEAP1 in serum, plasma, and cell culture supernatants, providing accurate and reproducible results for a wide range of research applications.KEAP1 is a critical regulator of the antioxidant response pathway, playing a key role in protecting cells from oxidative stress and maintaining cellular homeostasis. Dysregulation of KEAP1 has been implicated in a variety of diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing novel therapeutic approaches.
The Mouse KEAP1 ELISA Kit offers researchers a reliable and precise method for studying the expression levels of KEAP1 in mouse samples, facilitating the investigation of its role in various physiological and pathological processes. With its high sensitivity and specificity, this kit provides an invaluable tool for advancing our understanding of KEAP1 biology and its implications in disease.
Product Name:
Mouse Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit
Product Code:
MODL00760
Size:
96 Assays
Target:
KEAP1
Synonyms:
INrf2, KLHL19, Cytosolic inhibitor of Nrf2, Kelch-like protein 19
Detection Method:
Sandwich
Range:
78.1-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
