The Mouse KIM-1 (Kidney Injury Molecule-1) CLIA Kit is specifically designed for the accurate measurement of KIM-1 levels in mouse urine samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for research purposes.KIM-1 is a biomarker for kidney injury, with elevated levels indicating renal damage. Monitoring KIM-1 levels can provide valuable insight into kidney function and potential injury, making it a crucial tool for studying renal diseases and developing new treatment strategies.
The Mouse KIM-1 CLIA Kit is a reliable and convenient solution for researchers working in nephrology, toxicology, and drug development, allowing for the accurate assessment of kidney injury and the evaluation of potential therapeutic interventions.
Product Name:
Mouse KIM-1 (Kidney Injury Molecule 1) CLIA Kit
SKU:
MOES00421
Target:
Mouse KIM-1 (Kidney Injury Molecule 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse KIM-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse KIM-1. You can calculate the concentration of Mouse KIM-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
95-111
96-111
Average (%)
105
103
104
1:4
Range (%)
89-102
102-120
93-106
Average (%)
94
109
99
1:8
Range (%)
88-100
88-103
87-99
Average (%)
93
96
92
1:16
Range (%)
88-102
94-109
94-110
Average (%)
95
100
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-118
108
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
96-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.25
95.42
315.06
37.91
86.64
345.96
Standard deviation
3.97
8.43
30.66
4.68
10.06
26.12
C V (%)
9.62
8.83
9.73
12.35
11.61
7.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse KIM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse KIM-1 in samples. No significant cross-reactivity or interference between Mouse KIM-1 and analogues was observed.
