The Mouse LTF (LF Lactoferrin) CLIA Kit is a reliable and accurate tool for detecting LF Lactoferrin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and precise results, making it suitable for various research applications.LF Lactoferrin is a multifunctional protein known for its role in the immune system, iron metabolism, and antimicrobial activity. Its involvement in inflammatory responses and host defense mechanisms makes it an important biomarker for studying immune-related conditions, infectious diseases, and inflammatory disorders.
Overall, the Mouse LTF (LF Lactoferrin) CLIA Kit offers researchers a valuable tool for investigating the role of LF Lactoferrin in various biological processes and diseases, ultimately aiding in the development of potential therapeutic strategies.
Product Name:
Mouse LTF/LF (Lactoferrin) CLIA Kit
SKU:
MOES00422
Target:
Mouse LTF/LF (Lactoferrin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LF/LTF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LF/LTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LF/LTF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse LF/LTF. You can calculate the concentration of Mouse LF/LTF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
91-108
95-111
Average (%)
100
98
103
1:4
Range (%)
92-105
93-109
91-102
Average (%)
99
100
97
1:8
Range (%)
100-114
92-106
99-112
Average (%)
108
98
106
1:16
Range (%)
98-110
86-100
92-108
Average (%)
104
93
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
96-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.7
90.51
363.36
47.2
97.45
357.62
Standard deviation
5.86
8.64
39.5
5.93
7.24
22.71
C V (%)
12.82
9.55
10.87
12.56
7.43
6.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse LF/LTF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LF/LTF in samples. No significant cross-reactivity or interference between Mouse LF/LTF and analogues was observed.
